INSTRUMENTAL AND
BIOMEDICAL ANALYSIS – 4Th B-Pharm (50
Hours)
First Sessional
|
Sl.No. |
Chapter
No |
Topic |
Sub
Topic |
Hours |
|
01. |
Chapter
No-01 |
Chromatography |
Introduction
and classification, A) Column chromatography:
Adsorption column chromatography, development Techniques-Frontal analysis,
Displacement analysis and elution analysis. Factors affecting column
efficiency, Application, Partition Chromatography. B)
Thin layer chromatography: Introduction,
principle, technique, Rf values and application. C)
Paper Chromatography: Introduction,
Principles, Techniques, Development, methods and applications D)
Ion exchange chromatography: Ion
exchange materials, synthetic ion exchange resins, properties of ion
exchangers, mechanism, of ion exchange process affecting ion exchange,
application E)
Gas chromatography: Introduction,
theory, instrumentation-carrier gas, types of column, stationary phases in
gas liquid chromatography and gas solid chromatography. Detectors-Flame
ionization detector, electron capture detector, thermal conductivity
detector. Gas chromatogram, Derivatisation techniques like silylation, and
etherification, Programmed temperature gas chromatography, Application. F)
HPCL:
Introduction theory, instrumentation-Solvent treatment systems,
Pumps-Reciprocating and displacement pumps, Column, Detectors-UV detectors,
Flour metric detectors, Refractive index detectors and application. G)
Electrophoresis: Principles
of separation, equipment for paper and gel electrophoresis, Moving boundary
electrophoresis, Application. |
(01 Hours) (02 Hours) (01 Hours) (01 Hours) (03 Hours) (04 Hours) (02 Hours) (02 Hours) |
|
02. |
Chapter
No-02 |
Nephelometry
and Turbidimetric Analysis |
Theory,
General principles, Instrumentation and applications. |
(02 Hours) |
Second Sessional
|
01. |
Chapter
No-01 |
Absorption
spectroscopy |
Theory
of electronic, atomic and molecular spectra, Beer and Lambert’s law,
Derivation and Deviation, Application of Beer’s law to single component
analysis and multi component systems, Chromophores, Auxochromes, Bath chromic
shift, hypsochromic shift, Hyper chromic and hypo chromic effects, Effects of
solvent on absorption spectra. |
(05 Hours) |
|
02. |
Chapter
No-02 |
UV/Visible
spectroscopy |
Instrumentation
and working; Sources of radiation, Wavelength selectors; Filters-Prisms and
Gratings, Sample cell, Directors-Phototube, Photomultiplier tube, Barrier layer
cell and Silicon photo diode. |
(04 Hours) |
|
03. |
Chapter
No-03 |
IR
Spectroscopy |
Instrumentation, Working and Sample handling
methods, Sources of radiation, Monochromators, Simple cells and
detectors-Bolometer, Thermocouples, Golay cells. |
(03 Hours) |
|
04. |
Chapter
No-04 |
Application |
Measurement
of equilibrium constant and rate constant by spectroscopy, spectrophotometric
titration, IR frequency-structure correlation, study of characteristic
fundamental stretching vibrations of functional groups like Alcohol,
Carboxyl, Amide, esters, Amine, Aldehyde, Ketone and Phenol. |
(05 Hours) |
Third Sessional
|
01. |
Chapter
No-01 |
Flourimetric
analysis |
Theory,
concept of singlet and triplet electronic states, Internal and external conversions,
intersystem crossing factors affecting fluorescence, Quenching,
Instrumentation-Study of Flourimeter, spectrometric and Application. |
(03 Hours) |
|
02. |
Chapter
No-02 |
Flame
emission and atomic absorption spectrometry |
Theory,
Nebulization, flames, and flame temperatures, interference, flame
spectrometric techniques |
(03 Hours) |
|
03. |
Chapter
No-03 |
Electrometric methods : |
A)
Potentiometry : Electrochemical
cell, construction and working of reference electrode, Normal hydrogen
electrode, calomel electrode, Antimony electrode, Quinhydrone electrode,
Potentiometric titration, methods of detecting end point. B)
Conductometry : Introduction,
conductivity cell, conductometric titrations. Applications. |
(05 Hours) (02 Hours) |
|
04. |
Chapter
No-04 |
Quality assurance |
Introduction,
Sources of quality variation, control of quality variation, Validation
methods. |
(03 Hours) |