LUQMAN COLLEGE OF PHARMACY, GULBARGA

 

PHARMA MICROBIOLOGY AND BIOTECHNOLOGY – 2ND B-Pharm (75 Hours)

 

First Sessional

 

Sl.No.

Chapter No

Topic

Sub Topic

Hours

01

Chapter No-01

Introduction to the science of microbiology

Introduction to science and microbiology

Major division of microbial world, and relationship among them.

02 Hours

 

02

Chapter No-02

Classification and study of microbes

Study of bacteria- Anatomy

fungi, yeasts, actinomycetes,

Virus- Definition, classification and properties

Replication of virus

Cultivation of virus

mode of transmission and treatment of microbial diseases like- rickettsia and spirochetes

Cholera,Typhoid, Tuberculosis, Diphtheria,

Tetanus, Syphilis and AIDS.

10 Hours

03

Chapter No-03

Nutrition and culture of Microbes

Nutrition requirement- bacteria and virus, bacterial growth cycle

growth of aerobic and anaerobic bacteria and fungi.

and cultivation of. Study of different important media required for the Differential media, enriched media, and selective media, maintenance of laboratory culture.

06 Hours

04

Chapter No-04

Isolation and Identification

Different methods used in isolation and identification of bacteria with emphasis on different staining techniques and biochemical reaction. Counting of bacteria. Total and viable count. Bacteriological analysis of drinking water.

05 Hours

05.

Chapter No-05

Sterilizations

Detailed study of different methods of sterilization including their merits and demerits.

 

02Hours

 

 

 

Second Sessional

 

Sl.No.

Chapter No

Topic

Sub Topic

Hours

05

Chapter No-05 CONTINUED

Stessrilization

CONTINUED

Sterilization equipment, validation of sterilization. Sterilization methods for all pharmaceutical product. Detailed study of sterility testing of different pharmaceutical preparation.

09 Hours

06.

Chapter No-06

Disinfectants

Study of disinfectant, antiseptics, fungicidal and virucidal agent. Factors affecting their activities and mechanism of action. Evaluation of bactericidal, bacteriostatic, virucidal activities, evaluation of preservatives in pharmaceutical preparation.

06 Hours

 

07.

Chapter No-07

Introduction of genetics

Phenotypic and genotypic changes in bacteria, Mutations, genetic exchange in bacteria-transformation, transudation and conjugation.

04 Hours

 

08.

Chapter No-08

Genetic Engineering

Study of vector, plasmids, transposons, cosmids, Restriction endonucleases and DNA ligases. Steps involved in recombinant DNA technology and its application in the production of recombinint

06 Hours

 

 

 

 

Third Sessional

 

Sl.No

Chapter No

Topic

Sub Topic

Hours

08.

Chapter No-08 CONTINUED……….

Genetic Engineering

CONTINUED

product like Insulin, Hepatitis B Vaccine, growth hormone and interferon. Application of gel electrophoresis and southern blot techniques. Legislation and risks involved in the use of recombinant DNA products.

05 Hours

09.

Chapter No-09

Immunology

Antigens, structure and formation of antibodies, antigen antibody reaction. Diagnostic tests such as Shick’s test, Elisa test (HIV), VIDAL, Mantoux. And VDRL. Mehtods involved in production of vaccines- Polio mylitis, BCG, Typhoid, Diphtheria and tetanus toxoid and production of Sera-Diphtheria antitoxin.

08 Hours

 

10.

Chapter No-10

Fermentation

Introduction to fermentation technology. Methods of Penicillin, Streptomycin, Riboflavin and Cyanocobalamine.

04 Hours

 

11.

Chapter No-11

Microbiological Assays

Principles and methods of different microbiological assays including sensitivity testing, microbiological assay of Cephelexin, Sterptomycin and Vitamin B12. Standardisation of vaccines sera.

04 Hours

 

12.

Chapter No-12

Animal tissue culture

Techniques, nutritional requirements and characters of animal cell cultures. Hybridoma technology. Production vand application of monoclonal antibodies.

04 Hours