PHARMA
MICROBIOLOGY AND BIOTECHNOLOGY – 2ND B-Pharm
(75 Hours)
First Sessional
Sl.No. |
Chapter No |
Topic |
Sub Topic |
Hours
|
01 |
Chapter
No-01 |
Introduction
to the science of microbiology |
Introduction
to science and microbiology Major
division of microbial world, and relationship among them. |
02
Hours |
02 |
Chapter
No-02 |
Classification
and study of microbes |
Study
of bacteria- Anatomy fungi,
yeasts, actinomycetes, Virus-
Definition, classification and properties Replication
of virus Cultivation
of virus mode
of transmission and treatment of microbial diseases like- rickettsia
and spirochetes Cholera,Typhoid, Tuberculosis, Diphtheria, Tetanus,
Syphilis and AIDS. |
10
Hours |
03 |
Chapter
No-03 |
Nutrition
and culture of Microbes |
Nutrition
requirement- bacteria and virus, bacterial growth cycle growth of aerobic and anaerobic
bacteria and fungi. and cultivation of. Study of different
important media required for the Differential media, enriched media, and
selective media, maintenance of laboratory culture. |
06
Hours |
04 |
Chapter
No-04 |
Isolation
and Identification |
Different
methods used in isolation and identification of bacteria with emphasis on
different staining techniques and biochemical reaction. Counting of bacteria.
Total and viable count. Bacteriological analysis of drinking water. |
05
Hours |
05. |
Chapter
No-05 |
Sterilizations |
Detailed
study of different methods of sterilization including their merits and
demerits. |
02Hours |
Sl.No. |
Chapter No |
Topic |
Sub Topic |
Hours |
05 |
Chapter No-05 CONTINUED |
Stessrilization CONTINUED |
Sterilization
equipment, validation of sterilization. Sterilization methods for all
pharmaceutical product. Detailed study of sterility
testing of different pharmaceutical preparation. |
09 Hours |
06. |
Chapter
No-06 |
Disinfectants |
Study
of disinfectant, antiseptics, fungicidal and virucidal
agent. Factors affecting their activities and mechanism of action. Evaluation
of bactericidal, bacteriostatic, virucidal activities, evaluation of preservatives in
pharmaceutical preparation. |
06 Hours |
07. |
Chapter
No-07 |
Introduction
of genetics |
Phenotypic
and genotypic changes in bacteria, Mutations, genetic exchange in
bacteria-transformation, transudation and conjugation. |
04 Hours |
08. |
Chapter
No-08 |
Genetic
Engineering |
Study
of vector, plasmids, transposons, cosmids, Restriction endonucleases
and DNA ligases. Steps involved in recombinant DNA
technology and its application in the production of recombinint
|
06 Hours |
Third Sessional
Sl.No |
Chapter No |
Topic |
Sub Topic |
Hours |
08. |
Chapter
No-08 CONTINUED………. |
Genetic
Engineering CONTINUED |
product like Insulin, Hepatitis B
Vaccine, growth hormone and interferon. Application of gel electrophoresis
and southern blot techniques. Legislation and risks involved in the use of
recombinant DNA products. |
05 Hours |
09. |
Chapter
No-09 |
Immunology |
Antigens,
structure and formation of antibodies, antigen antibody reaction. Diagnostic
tests such as Shick’s test, Elisa test (HIV),
VIDAL, Mantoux. And VDRL. Mehtods involved in production of vaccines- Polio mylitis, BCG, Typhoid, Diphtheria and tetanus toxoid and production of Sera-Diphtheria antitoxin. |
08 Hours |
10. |
Chapter
No-10 |
Fermentation |
Introduction
to fermentation technology. Methods of Penicillin, Streptomycin, Riboflavin
and Cyanocobalamine. |
04 Hours |
11. |
Chapter
No-11 |
Microbiological
Assays |
Principles
and methods of different microbiological assays including sensitivity testing,
microbiological assay of Cephelexin, Sterptomycin and Vitamin B12. Standardisation
of vaccines sera. |
04 Hours |
12. |
Chapter
No-12 |
Animal
tissue culture |
Techniques,
nutritional requirements and characters of animal cell cultures. Hybridoma technology. Production vand
application of monoclonal antibodies. |
04 Hours |