APPLIED
BIOCHEMISTRY – 2ND B-Pharm (75 Hours)
First Sessional
|
Sl.No. |
Chapter
No |
Topic |
Sub
Topic |
Hours |
|
01. |
Chapter
No-01 |
Bio
chemical organization of the cell and transport processes across cell membranes. |
|
03Hours |
|
02 |
Chapter
No-02 |
Bio
Oxidation |
a)
Concept of free energy and its determination; redox
potential. b)
Energy rich compounds; ATP; Cyclic AMP; their biological significance. |
01 Hours 02 Hours (03 Hours) |
|
03 |
Chapter
No-03 |
Biological
Oxidation |
a)
Electron transport chains its mechanism and role. b)
Inhibitors and Uncouples of ETC c)
Oxidative phosphorylation d)
Substrate level phosphorylation and oxidative phosphorylation |
02 Hours 01Hours 01Hours 01Hours (05 Hours) |
|
04 |
Chapter
No-04 |
Enzymes
and Coenzymes |
a)
Definition; Nomenclature; IUB Classification. b)
Properties of enzymes; c)
Factors effecting enzyme activity; d)
Enzyme Kinetics (Michaelis plot; Lin Weaver Burke
Plot) e)
Enzyme Inhibition (with example) f)
Iso-enzymes g)
Enzyme Induction; repression h)
Application of enzymes i) Coenzymes, categories of
reaction requiring coenzymes; a)
Structure
of its coenzyme, and biochemical role of. b)
Vitamins-water
soluble, fat-soluble. |
01Hours 01Hours 02 Hours 02 Hours 01Hours 01Hours 01Hours 01Hours 04Hours (14 Hours) |
Second Sessional
|
Sl.No. |
Chapter
No |
Topic |
Sub
Topic |
Hours |
|
05 |
Chapter
No-05 |
Carbohydrate
metabolism |
a)
Introduction b)
Glycolysis c)
Glycogenesis glycogenolysis d)
TCA cycle; (Amphilbolic nature of TCA cycle) e)
Gluconeogensis f)
Various shuttle systems (glycerol phosphate; Malate
asparate. g)
HMP Shunt Pathway; h)
Uronic acid pathway and galactose
metabolism. i) Glucose tolerance test
and blood glucose regulation |
01Hours 02 Hours 01Hours 02 Hours 01Hours 01Hours 02 Hours 01Hours 01Hours (12 Hours) |
|
06. |
Chapter
No-06 |
Lipid
metabolism |
a)
Introduction b)
Beta-Oxidation of saturated fatty acids (palmitic
acid) c)
Beta-Oxidation of unsaturated fatty acids (a-linolinic
acid) d)
Formation and fate of ketones bodies e)
Cholesterol metabolism, f)
Biosynthesis of fatty acids (de novo) g)
Phospholipids and sphingolipids. |
01Hours 02 Hours 01Hours 01Hours 02 Hours 02 Hours 01Hours (10 Hours) |
Third Sessional
|
Sl.No. |
Chapter
No |
Topic |
Sub
Topic |
Hours |
|
07. |
Chapter
No-07 |
Amino
acid metabolism
|
a)
Amino acids definition, classification and significance. b)
General reaction of amino acids: Transamination,
Determination and decarboxylation of amino acids. c)
Urea cycle d)
Metabolism of sulphur containing amino acids. e)
Catabolism of tyrosine, tryptophan, phenylalanine. f)
Synthesis & significance of biologically important substances: creatine, histamine, 5-HT, dopamine, noradrenaline,
adrenaline. g)
Porphyrine, Bile Pigments; Hyperbilirubinemia. |
01Hours 02Hours 01Hours 01Hours 02Hours 02Hours 02Hours (11 Hours) |
|
08. |
Chapter
No-08 |
Nucleotides
and Nucleic acids |
a)
Introduction b)
Biosynthesis of purine nucleotides c)
Biosynthesis of pryimidine nucleotides d)
Catabolism of purines and pyrimidines e)
DNA Structure, signicificance as genetic material. f)
RNA types, structure and significance g)
Replication or DNAsynthesis h)
Mutation and repair of DNA i Transcription or RNA
synthesis j)
Genetic code k)
Translation or protein synthesis and its Inhibition. |
01Hours 01Hours 01Hours 01Hours 01Hours 01Hours 01Hours 01Hours 01Hours 01Hours 02Hours (12 Hours) |
|
09 |
Chapter
No-09 |
Principles
and significance for following Biochemical tests |
a)
Kidney function tests b)
Liver function tests c)
Lipid tests. |
02Hours 02Hours 01Hours (5 Hours) |